Abstract

Advances in neuroscience have significantly improved our understanding of neural circuit function and dysfunction in health and disease. Among the cutting-edge technologies contributing to this progress is in vivo two-photon microscopy, a powerful imaging technique that allows researchers to visualize and track the activity of neurons deep within the brain. This tool has provided unprecedented insights into how neural circuits are altered in various neurological disorders, such as Alzheimer’s disease, Parkinson’s disease, epilepsy, and autism spectrum disorders. By enabling direct observation of neuronal structures and signaling processes over time, two-photon microscopy is revolutionizing the study of neural circuit dysfunction and offering hope for the development of more effective treatments.