Journal of Biotechnology & Biomaterials
Open Access

Research Article

Transposon Induced Nitrogenase in Rhizobium japonicum Infecting Vigna radiata

¹Microbial Diversity Research Centre, Dr. DY Patil Biotechnology and Bioinformatics Institute, Pune, India

²Rajiv Gandhi Institute of Information Technology and Biotechnology, Bharati Vidyapeeth Deemed University, Pune, India

*Corresponding Author:

Mr. Ghatage AA, Ph.D.
Student, Dr. DY Patil Biotechnology and Bioinformatics Institute
Tathawade, Pune-411033, Maharashtra, India
Tel: 8806864547
E-mail: abhayghatage8@gmail.com

Received date:November 24, 2016; Accepted date: January 25, 2017; Published date: February 01, 2017

Citation: Ghatage A, Pandey R, Nawani N, Khetmalas M (2017) Transposon Induced Nitrogenase in Rhizobium japonicum Infecting Vigna radiata. J Biotechnol Biomater 7:252. doi:10.4172/2155-952X.1000252

Copyright: © 2017 Ghatage A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

The present study established a transposon mutagenesis procedure for Rhizobium japonicum forming symbiotic association with Mung bean (Vigna radiata). Suicide plasmid pko3 introduce the transposon Tn3 in to Rhizobium japonicum via Escherichia coli mediated conjugation and successfully generated 800 mutants with frequency of 3.7 × 10-6. These 800 mutants along with rifampicin resistant mutant and wild strain were screened for nodulation, which showed 100 mutants with pink coloured nodulation to mung bean plant. Out of 100 mutants 10 were promising for nitrogen fixation. The mutant AVR040 showed nitrogenase activity 12.4 μmol/h/mg fresh weight of nodule which is more than wild 9.61μmol/h/mg fresh weight of nodule, while AVR063 showed higher nitrogenase activity 19.4 μmol/h/mg fresh weight of nodule than AVR040.

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