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Trabecular Titanium Induces Osteoblastic Bone Marrow Stem Cells Differentiation
Vincenzo Sollazzo1, Annalisa Palmieri2, Ambra Girardi3, Francesca Farinella2 and Francesco Carinci2*1Orthopedic Clinic, University of Ferrara, Ferrara, Italy
2Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy
3Department of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy
- Corresponding Author:
- Prof. Francesco Carinci, MD
Deptartment of D.M.C.C.C, University of Ferrara
Corso Giovecca, 203,44100 Ferrara, Italy
Tel/Fax: 0039-0532-455582
E-mail: crc@unife.it
Received date: November 13, 2010; Accepted date: February 07, 2011; Published date: March 21, 2011
Citation: Sollazzo V, Palmieri A, Girardi A, Farinella F, Carinci F (2011) Trabecular Titanium Induces Osteoblastic Bone Marrow Stem Cells Differentiation. J Biotechnol Biomaterial 1:102. doi:10.4172/2155-952X.1000102
Copyright: © 2011 Sollazzo V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Pure titanium and titanium alloys are materials widely used in orthopedics and dental surgery because of their mechanical properties, chemical stability and biocompatibility. Although excellent clinical results have been shown, traditional porous metals have several inherent limitations (low volumetric porosity, relatively high modulus of elasticity and availability as a coating only). With the aim of going over these limits, improving the potentiality of osteointegration, a new highly porous titanium biomaterial (Trabecular Titaniumâ„¢, TT) has been developed. Because the molecular events due to TT and able to alter osteoblast activity to promote bone formation are poorly understood, expression of osteoblastic related genes in mesenchymal stem cells exposed to TT was investigated. The expression levels of bone related genes like RUNX2, SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1) and mesenchymal stem cells marker (CD105) were analyzed, using real time Reverse Transcription-Polymerase Chain Reaction.TT causes induction of bone related genes osteopontin (SPP1), osteocalcin (BGLAP) alkaline phosphatase (ALPL) and indicating the differentiation effect of this biomaterial on mesenchymal stem cells.The obtained results can be relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.
