Abstract

Tissue-resident memory T-cells (T_RM cells) are crucial for local immunity, especially in mucosal tissues. This study characterized T_RM cells’ phenotypic and functional profiles using flow cytometry and in vivo models. Results showed T_RM cells with a tissue-resident phenotype and potent effector functions. They were abundant in mucosal tissues and displayed tissue-specific gene expression. The protective role of T_RM cells against pathogen re-challenge and their potential in vaccine-induced immunity were demonstrated. These findings highlight T_RM cells as key players in local immunity and have implications for vaccine design and immunotherapy targeting mucosal infections.