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Hindi Review Article
The Promises, Methodological Discrepancies and Pitfalls in Measurement of Cell-Derived Extracellular Vesicles in Diseases
Alexander E Berezin1* and Richard E Mokhnach2
1Internal Medicine Department, Medical University, Zaporozhye, Ukraine
2Technical Department, National Technical University, Zaporozhye, Ukraine
- *Corresponding Author:
- Alexander E. Berezin
Internal Medicine Department
State Medical University of Zaporozhye
26, Mayakovsky av., Zaporozhye
UA-69035, Ukraine
Tel: +380612894585
E-mail: dr_berezin@mail.ru; aeberezin@gmail.com
Received date May 25, 2016; Accepted date June 11, 2016; Published date June 18, 2016
Citation: Berezin AE, Mokhnach RE (2016) The Promises, Methodological Discrepancies and Pitfalls in Measurement of Cell-Derived Extracellular Vesicles in Diseases. J Biotechnol Biomater 6:232. doi:10.4172/2155-952X.1000232
Copyright: © 2016 Berezin AE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Cell-derived extracellular vesicles (EVs) are heterogeneous population of phospholipid-based endogenously produced particles. EVs are detected in several biological fluids and tissues as biomarkers of diseases and target of medicines. The conventional approach for measuring the MPs is based on commonly used flow cytometry, fluorescent methods, and nano-particle tracking analysis that recognized as a gold standard, as well as Western blot analysis, dynamic light scattering, resistive pulse sensing, mass spectrometry-based proteomic methods and electron microscopy. However the definition of MPs using these techniques is yet under discussion. The aim of the review: to summarize the knowledge regarding detection and measurement of the EVs and define the balance between advantages and limitations of each contemporary analytical methods of EV assay.
