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Hindi Research Article
The Effects of 528 Hz Sound Wave to Reduce Cell Death in Human Astrocyte Primary Cell Culture Treated with Ethanol
Tohid Babayi and Gholam Hossein Riazi*Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
- *Corresponding Author:
- Gholam Hossein Riazi
Head of Biochemistry Department
Institute of Biochemistry and Biophysics-University of Tehran
Tehran, Iran
Tel: +98 21 61112473
E-mail: ghossein.riazi@gmail.com
Received date: June 20, 2017; Accepted date: July 05, 2017; Published date: July 12, 2017
Citation: Babayi T, Riazi GH (2017) The Effects of 528 Hz Sound Wave to Reduce Cell Death in Human Astrocyte Primary Cell Culture Treated with Ethanol. J Addict Res Ther 8:335. doi:10.4172/2155-6105.1000335
Copyright: © 2017 Babayi T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective: Alcohol consumption, because of damaging effects on various tissues including nerves system, causes a major problem in human societies. Nowadays, Usages of the non-pharmacological and non-invasive agents is common. Sound waves which are classified in non-invasive agent, beside on stimulation the auditory cells, also impact on non- auditory cells. It has been reported that the frequency of 528 HZ is related to the note MI and it shown some strange effects such as increasing the ability of DNA repair.
Materials and methods: In this paper, with MTT, LDH and ROS assays, it was evaluated the effect of ethanol on astrocytes primary cell culture which exposed to this frequency.
Results: The current results show that in IC50 of ethanol, the frequency of 528 Hz increased cells viability about 20% and the level of ROS production has been reduced up to 100%.
Conclusion: Therefore, the use of these sound waves can be useful to reduce the toxic effects of ethanol on astrocytes cells culture.
