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Hindi Research Article
SPR Based Fiber Optic Sensor for the Detection of Vitellogenin: An Endocrine Disruption Biomarker in Aquatic Environments
Sachin K. Srivastava1*, Roli Verma2, Banshi D. Gupta3, Isam Khalaila4 and Ibrahim Abdulhalim1,5
1Department of Electro optic Engineering and Ilse Katz Institute for Nanoscale Science and Technology, Ben Gurion University, Israel
2School of Chemistry, Reymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Israel
3Department of Physics, Indian Institute of Technology Delhi, India
4The Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University, Israel
5School of Materials Science and Engineering, Nanyang Technological University, Singapore
- Corresponding Author:
- Sachin KS
Department of Electro optic Engineering and Ilse Katz Institute for Nanoscale Science and Technology
Ben Gurion University, Beer Sheva-84105, Israel
Tel: +972-587-120-784
E-mail: sachinchitransh@gmail.com
Received Date: January 25, 2015; Accepted Date: May 27, 2015; Published Date: May 29, 2015
Citation: Srivastava SK, Verma R, Gupta BD, Khalaila I, Abdulhalim I (2015) SPR Based Fiber Optic Sensor for the Detection of Vitellogenin: An Endocrine Disruption Biomarker in Aquatic Environments Biosens J. 4:114. doi:10.4172/2090-4967.1000114
Copyright: © 2015 Srivastava SK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
We have fabricated a fiber optic SPR biosensor for the detection of vitellogenin (Vg), an endocrine disruption biomarker in aquatic environments. The sensor was fabricated by immobilizing anti-Vg antibodies on the sensor surface. Control experiments performed on another similar protein, fetuin and on a sensing probe without the anti-Vg antibody receptor confirmed the specificity of the sensor. The limit of detection of the sensor was found to be as small as 1 ng/ml in our experimental window. The sensitivity of the sensor was 0.48 nm/(ng/ml). This sensor can be utilized for remote monitoring of the water bodies for any endocrine disruption phenomena.
