Abstract

Single-cell analysis has emerged as a transformative approach in biomedical research, enabling the investigation of cellular heterogeneity and functionality at unprecedented resolution. This article reviews the key techniques employed in single-cell analysis, including microfluidics, mass spectrometry, and sequencing technologies. We explore the implications of these methods in understanding complex biological processes, such as development, disease progression, and therapeutic responses. Finally, we discuss future directions and challenges in the field.