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Hindi Research Article
Simultaneous Estimation of Aceclofenac, Paracetamol and Tizanidine in Their Combined Dosage Forms by Spectrophotometric and RP- HPLC Method
Chandrashekhar Narajji1*, Hardik R Patel1, Manohar D Karvekar2 and Suresh Babu AR31Dept of Quality Assurance, Acharya & B.M Reddy College of Pharmacy, Soldevanahali, Bengaluru -560 090, India
2Dept of Pharmaceutical Chemistry, Krupanidhi College of Pharmacy, Bengaluru- 560 035, India
3G7 Synergon Private Limited, Bengaluru– 560054, India
- *Corresponding Author:
- Dr. Chandrashekhar Narajji
Dept of Quality Assurance
Acharya & B.M Reddy College of Pharmacy
Soldevanahali, Bengaluru -560 090, India
E-mail: chandrashekharn@acharya.ac.in
Received date: July 13, 2011; Accepted date: October 03, 2011; Published date: October 07, 2011
Citation: Chandrashekhar N, Patel HR, Karvekar MD, Suresh BAR (2011) Simultaneous Estimation of Aceclofenac, Paracetamol and Tizanidine in Their Combined Dosage Forms by Spectrophotometric and RP- HPLC Method. J Anal Bioanal Tech 2:123. doi: 10.4172/2155-9872.1000123
Copyright: © 2011 Chandrashekhar N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
A rapid and sensitive RP-HPLC method with UV detection and UV spectrophotometric method for routine Pharmaceutical quality control of aceclofenac, paracetamol and tizanidine in tablets formulation was developed. Chromatography was performed by using Phenomenex-Luna C 18 (250 x 4.6 mm, 5μ) with a mobile phase containing Methanol: Water (90:10v/v), flow rate 1mL/min, wavelength at 256 nm. Linearity observed over the concentration range between 5-30μg/mL, 10-60μg/mL and 2-12μg/mL for aceclofenac, paracetamol and tizanidine respectively. The UV spectrophotometric method was performed at 277, 248 and 323 nm by derivative spectrophotometry with simultaneous equation method. The entire three drugs obey Beer’s law in the concentration range between 5-30 μg/mL, 2-16 μg/mL and 2-30 μg/mL respectively. The proposed methods were simple, rapid, precise, accurate and sensitive and can be used for routine quality control in pharmaceuticals.
