Abstract

Capillary Electrophoresis (CE) stands as a powerful analytical technique that has revolutionized the field of separation science and found diverse applications across various scientific disciplines. This abstract provides a succinct overview of the principles, methodologies, and applications of CE, highlighting its significance in modern analytical chemistry and biochemistry. The principles of CE are grounded in the differential migration of analytes under the influence of an electric field within a fused-silica capillary filled with a suitable buffer solution. Various modes of CE, including capillary zone electrophoresis, capillary isoelectric focusing, and capillary gel electrophoresis, offer distinct separation mechanisms that can be tailored to different analyte types and applications.