ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
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  • Research Article   
  • J Infect Dis Ther,
  • DOI: 10.4172/2332-0877.1000501

SARS-CoV-2 Rapid Antigen Test: Laboratory Validation of the Performance of POCT Antigen Test Kits of COVID-19

Zhang Lie1*, Yang Feng1 and Zhu Junzhe2
1Department of Nursing Sciences, Zhejiang Gongshang University, Hangzhou, China
2Department of Nursing Sciences, Wenzhou Medical University, Wenzhou, China
*Corresponding Author : Dr. Zhang Lie, Department of Nursing Sciences, Zhejiang Gongshang University, Hangzhou, China, Email: zhanglei@zjgsu.edu.cn

Received Date: May 10, 2022 / Published Date: Jun 13, 2022

Abstract

With the advent of deadly SARS-CoV-2, public health organizations and researchers emphasized the timely detection and identification of the virus to limit the spread. Different tests were designed to detect coronaviruses for timely and rapid identification, including Polymerase Chain Reaction (PCR) that detects the organism's genetic material. This research aims to validate the performance of Point-of-care testingantigen test kits of COVID-19, including the Lateral Flow Test (LFT) and the lateral Flow Immunofluorescent Assay (FIA) is intended for qualitative detection of SARS-CoV-2 nucleocapsid antigen. This evaluation involved six different rapid test kits, including Fia Test ATFNCG21040001, All Test ATNCP21060014, All Test ATNCP21060015, All Test ATNCP21060012, Abbott Panbio Cov-19 Ag Rapid Test, and Siemens Rapid COVID-19 Antigen Test. In addition, another study also compare the SARS-CoV-2 Antigen Rapid Test (COVID-19 Antigen Rapid Test) (Swab) manufactured by Hangzhou All Test Biotech Co., Ltd.; 2019-nCoV Antigen Test, manufactured by Guangzhou Wondfo Biotech Co., Ltd.; SARS-CoV-2 Rapid Antigen Test, manufactured by Roche, the molecular SARS-COV-2 assay (nucleic acid extraction using the automatic system Versant, followed by RT-PCR using the FDT SARS-CoV-2 kit) manufactured by Siemens from Germany that meets EU CE standards, to evaluate the clinical effectiveness of candidate kit. The Nasopharyngeal Swab (NPS) samples were collected and analysed by RT-PCR method. The specificity of all rapid test kits was greater than 99.9%, whereas the sensitivity ranged from 91 to >99%, which is also significant. The finding suggests the rapid antigen test can be used as an effective tool in controlling COVID-19 promptly. Moreover, these tests can also be conducted by a layman or at home for primary identification of COVID-19, which would limit the transmission of disease.

Keywords: COVID-19; Rapid antigen test; POCT Antigen test kit; Laboratory validation; Immunological assays; PCR; Lateral flow test; All Test

Citation: Lie Z, Feng Y, Junzhe Z (2022) SARS-CoV-2 Rapid Antigen Test: Laboratory Validation of the Performance of POCT Antigen Test Kits of COVID-19. J Infect Dis Ther 10: 501. Doi: 10.4172/2332-0877.1000501

Copyright: © 2022 Lie Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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