Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Research Article
Rhizodegradation of Hydrocarbon from Oily Sludge
| Asghari Bano1*, Asim Shahzad1 and Samina Siddiqui2 | |
| 1Department of Plant Scineces Quaid-i-azam University Islamabad, Pakistan | |
| 2Centre of Excellence in Geology University of Peshwar, Peshawar, Pakistan | |
| Corresponding Author : | Asghari Bano Department of Plant Scineces Quaidi- azam University Islamabad, Pakistan Tel: +92-051 9064-3137 E-mail: banoasghari@gmail.com |
| Received December 06, 2014; Accepted April 23, 2015; Published April 25, 2015 | |
| Citation: Bano A, Shahzad A, Siddiqui S (2015) Rhizodegradation of Hydrocarbon from Oily Sludge. J Bioremed Biodeg 6:289. doi:10.4172/2155-6199.1000289 | |
| Copyright: ©2015 Bano A, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
Related article at Pubmed Scholar Google |
|
Abstract
Rhizoremediation involves the use of plant growth promoting rhizobacteria (PGPR) to remove organic pollutants from terrestrial environment. The main objective of this study was to evaluate the efficiency of rhizoremediation through inoculation of hydrocarbon degrading bacterial strains with and without nutrients in the rhizosphere sporadically become contaminated with oily sludge. Bacterial strains viz. Bacillus cereus (Acc KF859972), Bacillus altitudinis (Acc KF859970), comamonas (Acc KF859971) and Stenotrophomonas maltophilia (Acc KF859973) were isolated from various oily waste pits of oil fields. Autoclaved soil was treated with oily sludge at 7:3 ratio and Alfalfa plant was inoculated with individual plant growth promoting rhizobacteria (PGPR) with and without diammonium phosphate and ammonium nitrate. The different saturates and total hydrocarbon was extracted by Soxhelt extraction and was analyzed by GC-FID at 0, 5 and 10 d of incubation. The inoculation 30% sludge with B. altitudinis accelerated the rate of degradation of n-alkanes and some methyl branched than un-inoculated soils. Oily sludge inoculated with B. altitudinis indicated that more than 80% of hydrocarbons were degraded at day 5 of incubation. No further degradation was observed till the end of the incubation period. B. altitudinis is capable of degrading straight chain hydrocarbons rapidly than any other strains. Alfalfa is a N-symbiotic plant and increased nutrient content under the rhizosphere promote degradation of n-alkanes. All other bacterial strains also showed significant interaction with alfalfa for the biodegradation of oily sludge but the rate of degradation remained slow than with B. altitudinis. Inoculation with B. altitudinis accelerated the rehabilitation process and within 5 days the rehabilitation occurs.
