Abstract

Banana (Musa species), is one of the most significant fruit crops grown widely in Ethiopia. It is extremely essential for ensuring food security in rural areas as well as for providing revenue and jobs. After rice, wheat, and maize, these are the next most significant food crops. India, China, Brazil, and Ecuador Field conservation of vegetatively propagated crops such as bananas are a key concern for germplasm curators. Nowadays, the slow growth storage (SGS) technique has become an economical in vitro method to preserve several plant species by controlling the growth and development of plantlets. As a procedure surface sterilized shoot tip meristem of about one-centimeter square explants were inoculated on Murashige and Skoog (MS) medium supplemented with plant growth regulator (PGR) and different types of plant growth retardant (PGR) such as mannitol, sorbitol, and sucrose.