Quantification of TUNEL Assay in Hippocampus of Diabetic Rats by MAT LAB: Comparison with Stereological Method
Received Date: Dec 06, 2011 / Accepted Date: Mar 19, 2012 / Published Date: Mar 21, 2012
Abstract
Quantification of immunostained sections is needed in order to reliably and accurately assesses the expression of cellular proteins in tissue. Here we describe a novel technique for quantification of TUNEL positive neurons in rat hippocampus. After uniform random sampling the slices were stained by TUNEL and counterstained by toluidine blue. Counting of TUNEL positive neurons performed by stereological method manually. The same sections were processed by MAT Lab software and the results compared together. The result showed there is no significant level of differences between the two methods (P<0.05). We believe merging image processing methods like MAT LAB and uniform random sampling facilitate the interpretation of study results.
Keywords: TUNEL; Stereology; MAT LAB; Hippocampus
Citation: Ahmadpour S,Younessi Heravi MA (2012) Quantification of TUNEL Assay in Hippocampus of Diabetic Rats by MAT LAB: Comparison with Stereological Method. J Clinic Experiment Pathol 2:108. Doi: 10.4172/2161-0681.1000108
Copyright: © 2012 Ahmadpour S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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