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Hindi Research Article
Purification and Antibacterial Activity of Marine Actinomycetes against Human and Fish Pathogens
Kumari Pushpa Rani TP* and Doss ADepartment of Microbiology, Kamaraj College, Thoothukudi, Tamilnadu, India
- *Corresponding Author:
- Kumari Pushpa Rani TP
Department of Microbiology
Kamaraj College
Thoothukudi, Tamilnadu
Tel: +91-461-2375988
E-mail: kpushparani24@gmail.com
Received date: November 14, 2016; Accepted date: November 24, 2016; Published date: November 30, 2016
Citation: Kumari Pushpa Rani TP and Doss A (2016) Purification and Antibacterial Activity of Marine Actinomycetes against Human and Fish Pathogens. J Marine Sci Res Dev 6:215. doi:10.4172/2155-9910.1000215
Copyright: © 2016 Kumari Pushpa Rani TP. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Many actinomycetes strains were isolated from soil samples of Thiruvananthapuram District, Kerala. The isolated strains were identified based on their morphological, cultural and biochemical characteristics. Totally thirty actinomycetes species were isolated and used for antibacterial activity. Out of thirty actinomycetes species, Streptomyces spp., showed the best level of antibacterial activity against human and fish pathogens. The bioactive compound protein was partially purified and tested for antibacterial activity. The partially purified bioactive compound was subjected for SDS-PAGE for protein profiling and band was obtained. The partially purified protein was allowed for High Performance Liquid Chromatography analysis and was tested for antibacterial activity against human and fish pathogens. The purified protein again subjected to SDS-PAGE analysis.
