Abstract

Pseudomonas putida has garnered significant attention as a promising candidate for industrial biocatalysis due to its robustness, metabolic diversity, and genetic tractability. This review explores the inherent biochemistry and transmetabolic potential of P. putida as a flexible framework for biocatalytic applications. With its diverse metabolic pathways, including aromatic compound degradation and fatty acid metabolism, P. putida exhibits inherent biochemistry that enables it to utilize a wide range of carbon sources. Moreover, its trans-metabolic potential allows for the engineering of novel biochemical transformations beyond its natural metabolic repertoire, facilitated by its amenability to genetic manipulation and synthetic biology approaches. Key strategies for enhancing P. putida's trans-metabolic capabilities include metabolic engineering, optimization of gene expression, pathway integration, and cofactor engineering. The applications of P. putida in industrial biocatalysis span environmental remediation, bioproduction of value-added chemicals, bioremediation of industrial wastes, and synthetic biology-driven biomanufacturing. However, challenges such as metabolic engineering complexity, strain stability, and scale-up remain to be addressed. By overcoming these challenges and leveraging its inherent biochemistry and trans-metabolic potential, P. putida holds promise as a versatile platform for sustainable and efficient biocatalytic processes in industrial settings.