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Hindi Research Article
PiPE, a Phytophthora-associated PAMPS from P. infestans, Binds to a Ca2+-Dependent Protein Kinase (CDPK) in Potato for the Induction of Hypersensitive Reaction
Naotaka Furuichi1,2,*, Kazutoshi Yokokawa2, Hisakazu Okamura2 and Masatoshi Ohta21Department of Agriculture, Niigata University, Niigata, 950-2181, Japan
2Graduate School of Science and Technology, and Niigata University, Niigata, 950-2181, Japan
- *Corresponding Author:
- Naotaka Furuichi
Department of Agriculture, Niigata University
Niigata, 950-2181, Japan
Tel: +81-25-262-7520
Fax: +81-25-262-7520
E-mail: nfuru@agr.niigata-u.ac.jp
Received date: August 21, 2013; Accepted date: January 07, 2014; Published date: January 09, 2014
Citation: Furuichi N, Yokokawa K, Okamura H, Ohta M (2014) PiPE, a Phytophthora-associated PAMPS from P. infestans, Binds to a Ca2+-Dependent Protein Kinase (CDPK) in Potato for the Induction of Hypersensitive Reaction. J Clin Exp Pathol 4:156. doi:10.4172/2161-0681.1000156
Copyright: © 2014 Furuichi N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
We report that PiPE, a Phytophthora-associated PAMPS (Pathogen Associated Molecular Patterns), induced generation of active oxygen species and hypersensitive cell death (HR) by treatment of potato tuber tissues, and that the PiPE gene from a species of Oomycete, Phytophthora infestans Mont (de Bary), was cloned. Interaction of a His-tagged PiPE from P. infestans with a His-tagged Ca2+-Dependent Protein Kinase (RiCDPK2) from potato cv. Rishiri (R1-gene) was investigated by using enzyme-linked immunosorbent assay with mouse monoclonal anti-PiPE Antibodies (Abs). We found that the PiPE and a Mycelical Homogenate (MH) from P. infestans can interact with His- RiCDPK2 in vitro. PiPE showed binding interaction with the His-fusion proteins from three other domains of RiCDPK2, indicating the existence of binding sites for PiPE of P. infestans on RiCDPK2. We suggest that after binding with the PiPE, RiCDPK2 may trigger signals that lead to the occurrence of HR in potato.
