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Hindi Research Article
Performance of Isolated Kocuria sp. SAR1 in Light Crude Oil Biodegradation
| Abdullah M. El Mahdi1, Hamidi Abdul Aziz1,2*, Salem S Abu Am1, Nour Sh. El-Gendy3 and Hussein Nassar3 | |
| 1School of Civil Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 NibongTebal, Penang, Malaysia | |
| 2Solid Waste Management Cluster, Engineering Campus, Universiti Sains Malaysia, 14300 Penang, Malaysia | |
| 3Egyptian Petroleum Research Institute, Nasr City, Cairo 11727, Egypt | |
| Corresponding Author : | Prof. Hamidi Abdul Aziz School of Civil Engineering Engineering Campus Universiti Sains Malaysia 14300 Nibong Tebal, Penang Malaysia Tel: + 60-45996215 Fax: +60-45941009 E-mail: cehamidi@eng.usm.my |
| Received June 09, 2015; Accepted July 06, 2015; Published July 08, 2015 | |
| Citation: El Mahdi AM, Aziz HA, Abu Am SS, El-Gendy NS, Nassar H (2015) Performance of Isolated Kocuria sp. SAR1 in Light Crude Oil Biodegradation. J Bioremed Biodeg 6:303. doi:10.4172/2155-6199.1000303 | |
| Copyright: © 2015 El Mahdi AM, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
In the current study; Kocuria sp. SAR1 was isolated from ‘Tobruk Refinery’ oil water pit, located along the eastern coast of Libya. The isolated bacterial strain SAR1 was characterized as an aerobic, Gram +ve, spherical-shaped, oxidase – but catalase +. Phenotypic characters and phylogenetic analysis based on the 16S rRNA gene of the isolate SAR1 showed that it was related to members of the Kocuria genus. The alignment of the 16S rRNA gene sequences of SAR1 with sequences obtained by doing a Blast searching revealed 96% similarity to Kocuria palustris strain TAGA27. Solid waste dates (SWD) and corn steep liquor (CSL) as agro-industrial products were performed to enhance the performance of Kocuria sp. SAR1 in crude oil biodegradation. During bacterial growth, high emulsifying activity to the presence of cells was observed, which is concluding the production of bio surfactant by strain SAR1. The bacterial strain showed removal ef?ciency of 68% and 70% of crude oil in 28 days when cultivated with 0.2% (w/v) of CSL and SWD, respectively. Crude oil metabolizing bacterium can secrete surfactants using agro industrial as substrates, which further enhance the hydrocarbon degradation.
