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Hindi Research Article
Pectinmethylesterase Production from mixed agro- wastes by Penicillium notatum NCIM. 923 in Solid-State fermentation
| Soumi Gayen* and Uma Ghosh | |
| Department of Food Technology & Biochemical Engineering Jadavpur University, West Bengal, Kolkata, India | |
| Corresponding Author : | Soumi Gayen Department of Food Technology & Biochemical Engineering Jadavpur University, West Bengal, Kolkata, India, E-mail: ssoumigayen@yahoo.co.in |
| Received April 16, 2011; Accepted June 15, 2011; Published June 17, 2011 | |
| Citation: Gayen S, Ghosh U (2011) Pectinmethylesterase Production from mixed agro- wastes by Penicillium notatum NCIM. 923 in Solid-State fermentation. J Bioremed Biodegrad 2:119. doi:10.4172/2155-6199.1000119 | |
| Copyright: © 2011 Gayen S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
The enzyme pectinmethylesterase (PME; EC 3.1.1.11) catalyzes the hydrolysis of the methyl ester groups from pectin and has been found in plants as well as in pathogenic fungi and bacteria. PME is of significance to the citrus industry because it has been established as the causative agent for juice clarification and gelation of frozen concentrates. The fruit processing industries produce a large amount of waste material, which poses considerable disposal problems and ultimately leads to pollution. Dried citrus peel is rich in carbohydrates, proteins and pectin; pectin acts as the inducer for production of pectinolytic enzymes by microbial systems. Thus, in the present study, dried citrus peel and wheat bran was used as substrate for the production of pectin methyl esterase (PME; EC 3.1.1.11) by fungus Penicillium notatum . Maximum enzyme activity was obtained with 1:1(w/w) substrate ratio, which gives a solid mass of initial pH 5.5, when incubated at 30°C for 120 h at 1:1 (w/v) initial moisture content ratio under static condition.
