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  • Research Article   
  • Cell Mol Biol, Vol 68(5): 240
  • DOI: 10.4172/1165-158X.1000240

Pancreatic Juice Pathological Microenvironment Induces Apoptosis of Human Pancreatic Duct Epithelial Cells through Autophagy by Activating p38 MAPK Signaling Pathway

Jin Ping Shi1, Tong Tong Dong1, Liang Ping Chen1, Qian Qian Hao1, Wei Jie Yao2 and Zuo Zheng Wang2*
1Department of Hepatobiliary Surgery, Clinical College of Ningxia Medical University, Ning Xia 75004, China
2Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Ning Xia 75004, China
*Corresponding Author : Zuo Zheng Wang, Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Ning Xia 75004, China, Tel: +86 13895009292, Email: wangzuozheng2008@sina.com

Received Date: Jul 18, 2022 / Accepted Date: Aug 11, 2022 / Published Date: Aug 11, 2022

Abstract

Background: Acute pancreatitis is a common acute abdominal disease in clinical practice, the essence of which is the inflammatory reaction to the pancreas. Patients with rapid progression of the disease may have local or systemic inflammatory reaction. In this pathological process, the pancreatic duct mucosal barrier function is impaired. However, the specific mechanism is still unclear.

Methods: Naso-pancreatic tube drainage was performed in the early stage of severe acute pancreatitis, pancreatic juice was extracted from drainage bag of patients within 24 hours after operation. Pancreatic juice extracted was used to stimulate Human Pancreatic Ductal Epithelial (HPNE) cells, and the proliferation of HPNE cells was detected by Cell Counting Kit-8 (CCK8) and 5-Ethynyl-2’-Deoxy Uridine (EDU) methods. Annexin-V/PI apoptosis detection kit was used to detect cells apoptosis. Fluorescence microscope was used to observe the occurrence of autophagy flux in pancreatic juice pathological microenvironment. After HPNE cells were treated with 3-Methyl Adenine (3-MA), and detected the expression of apoptotic proteins by Western blotting. HPNE cells were treated with p38 MAPK signaling pathway inhibitors (SB203580) for 4 hours, and then detected the expression of apoptosis and autophagy proteins by western blotting.

Results: The proliferation of HPNE cells was inhibited, and apoptosis of HPNE cells occurred in pancreatic juice pathological microenvironment. Besides, the pancreatic juice pathological microenvironment induced autophagy in HPNE cells. Interference with autophagy reduced the apoptosis of HPNE cells. Interference of p38 MAPK signaling pathway with SB203580 inhibited autophagy and reduced the apoptosis of HPNE cells.

Conclusion: In the pancreatic juice microenvironment of severe acute pancreatitis, the activation of p38 MAPK signaling pathway induced excessive autophagy and apoptosis of HPNE cells, and inhibition of p38 MAPK signaling pathway reduced the apoptosis of HPNE cells.

Citation: Shi JP, Dong TT, Chen LP, Hao QQ, Yao WJ, et al. (2022) Pancreatic Juice Pathological Microenvironment Induces Apoptosis of Human Pancreatic Duct Epithelial Cells through Autophagy by Activating p38 MAPK Signaling Pathway. Cell Mol Biol, 68: 240. Doi: 10.4172/1165-158X.1000240

Copyright: © 2022 Shi JP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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