Abstract

Reversed-phase chiral chromatography is a powerful analytical technique used for the separation and analysis of enantiomers in hydrophobic environments. Enantiomers are mirror-image isomers with distinct biological activities, despite possessing identical physical and chemical properties. Reversed-phase chiral chromatography operates on the principle of differential interaction and partitioning of enantiomers between a hydrophobic chiral stationary phase and the mobile phase. The stationary phase is typically modified with chiral selectors that selectively interact with one enantiomer over the other based on their spatial arrangement. Reversed-phase chiral chromatography offers several advantages, including broad applicability to hydrophobic and moderately polar compounds, compatibility with various detection techniques, and high resolution and sensitivity. It finds applications in various fields such as pharmaceuticals, natural products, and fine chemicals. In pharmaceutical research and development, it plays a crucial role in enantiomeric separation, determination of enantiomeric purity, and characterization of chiral impurities. In the analysis of natural products, it aids in the identification of enantiomeric compounds and their biological activities. In the production of fine chemicals, it ensures the synthesis of enantiomerically pure compounds for various applications.