Abstract

Due to the complexity and individual problems presented by each biological matrix, sample preparation is regarded as the bottleneck phase in bio analysis. An essential stage in every bio analytical technique is competent sample preparation to extract the required analyses and remove superfluous components. In the processing of bio analytical samples, the matrix effect is a significant barrier that has drawn a lot of attention. Over the past ten years, novel sample preparation methods have gained popularity due to their advantages over traditional methods in terms of accuracy, automation, simplicity of sample preparation, storage, and shipment. Our goal is to give a thorough overview of recent advancements in a variety of bioanalytical sample preparation methods for chromatographic and spectroscopic analyses. With the help of preferred instances, it is also indicated how these procedures have attracted a lot of attention in bio analytical research during the past 10 years. The focus is mostly on current developments in sorbent-based micro extraction techniques and other modern trends in bioanalytical sample preparation.