Abstract

Zone electrophoresis is a widely utilized technique in biochemistry and molecular biology for the separation of proteins based on their charge and size. This study focuses on optimizing critical parameters such as buffer composition, pH, applied voltage, and gel matrix concentration to enhance the resolution of protein separation. A systematic approach was employed to evaluate the impact of these variables on the separation efficiency and resolution. Results indicate that specific combinations of buffer systems and gel concentrations significantly improve protein separation profiles. The findings highlight the potential of optimized zone electrophoresis as a robust analytical tool in proteomics, diagnostics, and biopharmaceutical applications.