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Hindi Research Article
Optimization of Phenol Degradation Using Pseudomonas aeruginosa (MTCC 7814) by Plackett-Burman Design and Response Surface Methodology
| Pandimadevi M*, Venkatesh Prabhu M and Vinod Kumar V | |
| Department of Biotechnology, SRM University, India | |
| Corresponding Author : | Pandimadevi M Department of Biotechnology SRM University, India Tel: +91 9444145987 E-mail: pandimadevi.m@ktr.srmuniv.ac.in, pandimadevi2008@gmail.com |
| Received August 13, 2014; Accepted November 25, 2014; Published November 28, 2014 | |
| Citation: Pandimadevi M, Venkatesh Prabhu M, Vinod Kumar V (2014) Optimization of Phenol Degradation Using Pseudomonas aeruginosa (MTCC 7814) by Plackett- Burman Design and Response Surface Methodology. J Bioremed Biodeg 5:261. doi:10.4172/2155-6199.1000261 | |
| Copyright: © 2014 Pandimadevi M, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
In the present study, statistical screening and optimization of phenol degradation was done by Pseudomonas Aeruginosa (MTCC 7814), which can utilize phenol as a sole carbon and energy source. Nine medium variables Phenol, K2HPO4, K2H2PO4, MgSO4, (NH4)2SO4, MnSO4, FeSO4, NaCl and H3BO3 were screened by Plackett-Burman (PB) method. K2HPO4, (NH4)2SO4, MnSO4 and phenol were significant in PB method. Central composite design (CCD) and Response Surface Methodology (RSM) were applied to optimize the significant variables identified from the PB experiment. Statistical analysis of the experimental results showed optimal values were found to be KH2PO4 0.025 g/L, (NH4)2SO4 0.45 g/L, MnSO4 0.05 g/L and phenol 1 g/L with maximum phenol degradation of 83.86%. Maximum phenol degradation of 81.62% was observed in the validation experiment. This experimental result explained the model was fitted 97.32 % as compare with the result predicted by response surface method. This study indicated the excellent ability of Pseudomonas Aeruginosa to degrade phenol of high concentration.
