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Optimization of Fertilization Success in the Bivalve Mollusk Tivela mactroides under Laboratory Conditions
Yajaira García de Severeyn1*, Ana Villasmil1, Héctor Severeyn2, Félix Morales3 and Marynes Montiel41Universidad del Zulia, Facultad Experimental de Ciencias, Dpto. de Biología, Laboratorio de Cultivo de Invertebrados Acuático, Venezuela
2Universidad del Zulia, Facultad Experimental de Ciencias, Dpto. de Biología Laboratorio de Sistemática de Invertebrados Acuáticos, Venezuela
3Universidad del Zulia, Facultad Experimental de Ciencias, Dpto. de Biología Laboratorio de Oceanografía, Venezuela
4Universidad del Zulia, Facultad Experimental de Ciencias, Dpto. de Biología, Unidad de investigaciones de Microbiología Ambiental
Maracaibo, Zulia, Venezuela
- *Corresponding Author:
- Yajaira García de Severeyn
Universidad del Zulia, Facultad Experimental de CienciasDpto. de Biología
Laboratorio de Cultivo de Invertebrados Acuático, Venezuela
Tel: 58-261-7434136
Fax: 58-261-7434136
E-mail: ygsevereyn@yahoo.com
Received date: September 04, 2013; Accepted date: December 23, 2013; Published date: December 30, 2013
Citation: Severeyn YG, Villasmil A, Severeyn H, Morales F, Montiel M (2013) Optimization of Fertilization Success in the Bivalve Mollusk Tivela mactroides under Laboratory Conditions. J Marine Sci Res Dev 4:142. doi:10.4172/2155-9910.1000142
Copyright: © 2013 Severeyn YG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
With the objective to produce seed in an aquaculture facility it was studied under laboratory conditions, the effect of sperm concentration and contact time between gametes to optimized fertilization success of the bivalve mollusk Tivela mactroides. Mature ovocites and spermatozoids gotten through gonads dissection of adult mature male and female clams were artificially combined to produce fertilization. Solutions of 5.5×106 ovocites were fertilized with 10 different sperm concentrations. Results indicate that the percentage of succeed fertilization was low, highly variable and relying on the sperm concentration. The maximum mean percentage of fertilization was 22% between 1.3×107 and 1.7×107 spermatozoid/ml. A strong fall in fertilization success was observed at sperm concentrations lower than 106 spermatozoid/ml. The time lapsed after the first contact for fertilization that produced the highest fertilization rate was three hours, at sperm concentrations higher than 107 spermatozoids/ml. The highest fertilization percentage found in this study falls between the ranges reported for other bivalve mollusk species.
