Abstract

DNA microarrays have revolutionized genomics by enabling high-throughput analysis of gene expression. However, conventional microarrays often suffer from limited sensitivity and signal-to-noise ratios, which can impede the accurate detection of low-abundance transcripts. In Situ Catalytic Pattern Multiplication (ICPM) is a transformative technique that addresses these limitations by combining microarray technology with catalytic signal amplification. This abstract provides an overview of ICPM’s principles and its applications in DNA microarrays. ICPM employs catalytic reactions to amplify detection signals, significantly enhancing sensitivity and reducing background noise. This innovation enables researchers to detect subtle gene expression changes associated with diseases, identify potential drug targets, and personalize medical treatments based on an individual’s genetic profile. Furthermore, ICPM facilitates multiplexed analysis, allowing for the simultaneous investigation of multiple gene targets in a single experiment.