Abstract

When cells in the intestinal epithelium are subjected to heat and hypoxia, compromising the epithelial integrity of the epithelium, a (heat) stress response is triggered. It appears that several categories of nutritional components have beneficial effects on maintaining the integrity of the intestinal epithelium when it is stressed. When Caco-2/HT- 29 cells were exposed to heat or hypoxia, the expression of claudin-3 and zonula occludens-1 (ZO-1) was prevented from decreasing by non-digestible oligosaccharides (GOS), fructo-oligosaccharides (FOS), chitosan oligosaccharides (COS), antioxidants (-lipoic acid (ALA), resveratrol (RES), amino acids By reducing MDA production caused by heat or hypoxia, COS, RES, and EPA demonstrated anti-oxidative stress effects, while Arg further increased HSP-70 expression caused by heat or hypoxia. 4) Several nutritional components may be interesting subjects for future clinical trials and in vivo studies on heat stress and hypoxia-related gastrointestinal disorders, based on the findings of this study. These conditions include heat stress and hypoxia.