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Hindi NPIASA a Novel Peptide Prevents Sh-Sy5y Neuroblastoma Cells Against Rotenone-Induced Mitochondrial Dysfunction, Oxidative Stress and Apoptosis
*Corresponding Author: Arulkumar K Arul, Department of Biotechnology, Madanapalle Institute Of Technology and Science, Andhra Pradesh, India, Tel: 9629860719, Email: arulkumarmanivannan@gmail.com
Copyright: © 2021 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The present research was designed to explore the neuroprotective effect of NPIASA against rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis in a SH-SY5Y human neuroblastoma cellular model. The cells were divided into four experimental groups (control, rotenone (100 nM), NPIASA (5) + rotenone (100 nM), NPIASA (5) alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. In SHSY5Y cells, rotenone induced cytotoxicity, oxidative stress and mitochondrial dysfunction whereas pre-treatment of NPIASA attenuated the rotenone toxicity. Besides, rotenone induced the cytotoxicity by up-regulating caspases -3, -6, -8, -9 expressions and down regulating Bcl2 expression. NPIASA pre-treatment reversed the toxicity effects induced by rotenone in cells. Collectively, our results proposed that NPIASA mitigated the rotenone-induced oxidative stress, mitochondrial dysfunction and apoptosis. However, additionally pre-clinical studies are warranted in rodents to use NPIASA as a revitalizing therapeutic agent for PD in future.
