Novel saccharification of lignocellulosic biomasses using whole broth enzymes: Towards on-site manufacturing (osm) of enzymes for production of 2nd generation ethanol from eucalyptus wastes in biorefinery configurations.
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Abstract
Saccharification of lignocellulosic biomasses using enzymatic hydrolysis has been proven to be an attractive route for the production of second generation (2G) ethanol from abundant low-cost renewable feedstocks such as eucalyptus wastes. Nevertheless, approximately 30-40% of the overall production cost of ethanol from biomasses might be attributed to the price of outsourced enzymes, which incorporates significant cost parameters related to the need for purification, stabilization, packing, transportation, storage and conservation, among others.