Novel approach to prepare biopolymers based Nano-crystals
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Copyright: © 2018 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
New techniques developed to prepare biopolymer Nano crystals from easily available starch and protein source. Though Nano crystals preparation has been reported by acid hydrolysis in case of starch Nano crystals, some adverse effects may be noted when used as fillers. Starch crystals are prepared by addition of plasticizer further followed by homogenization and ethanol wash followed by freeze drying. Particle size of pea starch observed by Transmission Electron microscopy is 50-100nm and the morphology is determined by Scanning electron microscopy. Certain repetitive solubilisation and crystallization method has been tried out in case of protein Nano crystals. Results from Scanning electron microscopy and Transmission Electron microscopy can be noted that the particle size of first stage protein isolate is of 870nm whereas after optimization of last stage (final solubilisation and crystallization) results in 350nm. Thus there is a tendency of size reduction in case of protein crystals