Abstract

Nanotechnology is an emerging field of science which encompasses fabrication of nanoparticles that possess distinct morphological properties with enhanced functionality. Nanobiotechnology plays a fundamental role in the development of a bio-based green approach for their synthesis. This method is not only ecofriendly but provides a clean and nontoxic way for the fabrication and assembly of different nanoparticles. Microorganisms such as fungal strain of Aspergillus niger could be employed to produce silver nanoparticles. By utilizing fungal biomass and its LCF (Live cell filtrate), colloidal AgNPs were synthesized through reduction of silver metal ions by nitrate reductase enzyme present in the fungal filtrate. Color change from yellow to brownish black of the experimental flask incubated with silver nitrate solution at 25°C for 48 h indicates silver nanoparticle formation. UV vis spectrometry results showed that the maximum absorbance was obtained at 420 nm and 400 nm.

Keywords: Nanotechnology, Silver nanoparticles (SNPs), Aspergillus niger