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  • Research Article   
  • Cell Mol Biol,
  • DOI: 10.4172/1165-158X.1000205

Mycobacterium tuberculosis HtdY, a Novel Immunostimulatory Antigen, Drives Th1-type T Cell Immunity via TLR4-mediated Activation of Dendritic Cells.

Xiaoli Yu1, Xuefang Li1, Peng Hu1, Yang Li1, Fang Zhou1, Ke Zhang1*, Xinqian Wang2, Danni Wang4 and Shulin Zhang1,3,4
1College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, PR China
2MDPI (Multidisciplinary Digital Publishing Institute) Science and Technology Services Co. Ltd., Wuhan 430064, PR China
3Shanghai Public Health Clinical Centre, Shanghai 201508, PR China
4Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China
*Corresponding Author : Ke Zhang, College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, PR China, Tel: (+86)027-83956793-201, Email: kzhang@whpu.edu.cn

Received Date: Aug 03, 2021 / Accepted Date: Sep 07, 2021 / Published Date: Sep 14, 2021

Abstract

Mycobacterium tuberculosis (MTB), the etiological factor of tuberculosis (TB), is among the most successful of intracellular pathogen that regulates the host immune response. Cellular immune responses perform a pivotal function in host defence against MTB. Thus, it is vital to understand the antigens which drive immune protection, especially Th1-type cellular immunity. The role of 3-hydroxyacyl-l-thioester dehydratase (HtdY, Rv3389c) of MTB in immunological protection was observed in dendritic cell (DC) activation and T cell immunity herein. Recombinant HtdY was applied for inducing maturation and activation of DCs obtained from murine bone marrow. TLR4 and TLR2 knockout mice and pharmacological inhibitors were used to investigate the mechanism by which HtdY activates DCs. MLR assay was performed to characterize HtdY activity with respect to DC activation and T cell polarization. The alteration of cytokines secretion by human PBMCs elicited by HtdY was observed. We found that HtdY prompted DC maturation and activation through augmenting the expression of surface molecule CD80, CD86 and MHC II, and the release of pro-inflammatory cytokines including IL-1β, IL-6, IL-12 and TNF-α from DCs. HtdY-drived DC activation involved TLR4 activation and mitogen-activated protein kinases (MAPKs) signaling pathway. DCs treated with HtdY induced naïve CD4+ T cells to produce IFN-γ. Human PBMCs had higher TNF-α and lower IL-10 response to HtdY in active TB group than that in control group (p<0.01). Our results indicated that HtdY possesses potential to drive Th1-type cellular immunity by TLR4- mediated activation of DCs.

Keywords: Mycobacterium tuberculosis; HtdY; DC maturation; TLR4; Th1 polarization; IL-10

Citation: Yu X, Li X, Hu P, Li Y, Zhou F, et al. (2021) Mycobacterium tuberculosis HtdY, a Novel Immunostimulatory Antigen, Drives Th1-type T Cell Immunity via TLR4-mediated Activation of Dendritic Cells. Cell Mol Biol 67: 205. Doi: 10.4172/1165-158X.1000205

Copyright: © 2021 Yu X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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