ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Infect Dis Ther 2019, Vol 7(1): 396
  • DOI: 10.4172/2332-0877.1000396

Multilocus Sequence Typing and Molecular Detection of Phenol-soluble Modulin in Biofilm-positive Staphylococcus epidermidis Isolated from Paediatric Blood Culture

Grace A John- Ugwuanya1*, Stephen K Obaro1, Busayo O Olayinka2, Josiah A Onaolapo2, Fatimah Hassan Hanga3, Huda Munir3 and Paul D Fey4
1International Foundation against Infectious Diseases in Nigeria, Abuja, Nigeria
2Department of Biotechnology, Ahmadu Bello University, Zaria, Nigeria
3Murtala Mohammed Specialist Hospital, Kano, Nigeria
4Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA
*Corresponding Author : Grace A John- Ugwuanya, International Foundation against Infectious Diseases in Nigeria, Abuja, Nigeria, Tel: +3908713554026, Email: graceamy2k@yahoo.com

Received Date: Feb 12, 2019 / Accepted Date: Feb 22, 2019 / Published Date: Feb 28, 2019

Abstract

Aim: Staphylococcus epidermidis is a significant coagulase-negative staphylococci obtained from blood culture samples. However, there is limited information about phenol-soluble modulin (PSM), which is associated with virulence in S. epidermidis and its genetic relatedness in Nigeria. This study observed the presence of phenolsoluble modulin mec (psm-mec) gene and the multilocus sequence typing (MLST) of biofilm-positive Staphylococcus epidermidis (BPSE).

Method: Twenty-two biofilm-positive S. epidermidis isolates obtained from paediatric blood culture at three hospitals in north-west and north-central Nigeria were evaluated for the molecular detection of the psm-mec gene using conventional polymerase chain reaction (PCR). The biofilm formation was previously assessed by molecular detection of the intercellular adhesion (icaA) gene and the methicillin resistance using cefoxitin disk agar diffusion. Internal fragments of the respective seven housekeeping genes was sequenced for 21 BPSE strains and matched with the central MLST database.

Results: Out of 22 BPSE, only 4.5% had the psm-mec gene and it was methicillin resistant. About 91% methicillin resistance was observed among the psm-mec negative BPSE strains. Twenty-one BPSE strains were sequence type 1 (ST1) based on the MLST analysis of the seven housekeeping genes.

Conclusion: Considering the very low presence of psm-mec gene, the BPSE may be carrying the ß-type PSMs related to biofilm formation and dissemination, and not the cytolytic a-type PSMs common in the aggressive form of S. epidermidis. Phenol-soluble modulin methicillin resistance island-encoded peptide toxin is involved in sepsis related methicillin-resistant S. epidermidis. The strains are genetically related to each other.

Keywords: Phenol-soluble modulin; Methicillin resistance; Staphylococcus epidermidis ; Biofilm-positive; Multilocus sequence typing

Citation: John-Ugwuanya GA, Obaro SK, Olayinka BO, Onaolapo JA, Hassan-Hanga F, et al. (2019) Multilocus Sequence Typing and Molecular Detection of Phenol-soluble Modulin in Biofilm-positive Staphylococcus epidermidis Isolated from Paediatric Blood Culture. J Infect Dis Ther 7: 396. Doi: 10.4172/2332-0877.1000396

Copyright: © 2019 John-Ugwuanya GA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Top