Abstract

This study interprets the characteristic features of vitrification in plant tissue culture. Vitrescence is physiological decrepitude that hovers morphological and ecological aspects at its back. The foremost determinants of hyperhydricity are both external and internal factors. These antecedents may be the imbalancing of compounds in culture media or other agents like light, temperature and water potential. Abnormal state of leaves, stem, disruption of vascular bundle, hyperhydricity of cells, loss of chlorophyll, adverse effect on cytoplasm and depletion of lignin in cell wall of micropropagated plants are some of the consequences of glassiness. Extreme level of temperature, humidity, low light intensity, high ammonium and chlorine in culture media, low agar and low level of cellulose with lignin, hyperhydric condition and imbalance in culture media are primary roots of vitrification. Passable check and balance of hormones, organic and inorganic compounds, supporting agents, atmospheric factors and proper exchange of gases in container with environment are capital remedies to reverse the hyperhydric malformation. The proper concernment of plant tissue culturists is highly wanted to eliminate this state during tissue culture. Further research is executing in this area of study.