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Hindi Research Article
Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays
Julio E Rincon1, Fabio Diaz Santillan1, Pedro M Palermo Infante2, Douglas M Watts2 and Thomas Boland1*1Biomedical Engineering, University of Texas at El Paso, El Paso, TX, USA
2Biological Sciences, University of Texas at El Paso, El Paso, TX, USA
- Corresponding Author:
- Boland N
Biomedical Engineering
University of Texas at El Paso
El Paso, TX, USA
Tel: 915-747-7992
E-mail: tBoland@utep.edu
Received date: April 18, 2017; Accepted date: June 06, 2017; Published date: June 13, 2017
Citation: Rincon JE, Santillan FD, Infante PMP, Watts DM, Boland T (2017) Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays. J Biotechnol Biomater 7:260. doi:10.4172/2155-952X.1000260
Copyright: © 2017 Rincona JE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Abstract
A new molecular imprinting technique was developed for molecularly imprinted polymer particles (MIPs). Particles were synthesized using organic silane chemistries by a sol-gel process, where the relative amount of active monomers was complementary matched to the relative amount of surface charges of the West Nile antibody template. Synthesized MIPs showed specific binding to affinity purified polyclonal West Nile antibodies (WNA) with a loading capacity of 80 μg/mg, while MIPs absorbed non-specific proteins at a loading capacity of 28 μg/mg. A dissociation constant of Kd=57.45 μM was measured from the binding isotherms. MIPs selectively absorbed 27 times more WNA than either albumin or immunoglobulin, while MIPs absorbed 16 times more WNA than nonimprinted particles (NIPs). Finally, fluorescently labeled MIPs were incubated in a high bind 96 well plate previously loaded with template, albumin, or immunoglobulin as an immunoassay test. Fluorescent MIPs significantly bound more to wells with WNA than any other control. Thus, the development of new affordable and robust immunoassays with MIPs would be possible in the future.
