Abstract

This article explores the molecular approaches employed in the detection of Herpes Simplex Virus (HSV) infections, focusing on their significance in accurate diagnosis and clinical management. Molecular techniques, such as Polymerase Chain Reaction (PCR), Loop-Mediated Isothermal Amplification (LAMP), nucleic acid hybridization, Next-Generation Sequencing (NGS), Digital PCR (dPCR), and multiplex PCR panels, have revolutionized the field of HSV diagnostics. These methods offer high sensitivity, specificity, and rapid results, aiding in the differentiation of HSV types, viral load quantification, and monitoring disease progression. The application of these molecular tools enhances our ability to provide timely interventions, prevent transmission, and improve patient outcomes in HSV infections.