Abstract

Miniaturized chromatographic techniques represent a significant advancement in analytical chemistry, offering enhanced efficiency, sensitivity, and reduced sample and solvent consumption compared to traditional methods. This article provides a comprehensive overview of various miniaturized chromatographic approaches, including microcolumn chromatography, microfluidics, and nanochromatography. We discuss the methodologies employed, their applications across various fields such as pharmaceuticals, environmental analysis, and food safety, and the advantages they confer over conventional techniques. Additionally, we explore the challenges and future perspectives associated with miniaturized chromatography. Through case studies, we highlight practical implementations and their impact on analytical performance. The conclusion emphasizes the potential of miniaturized chromatographic techniques to revolutionize analytical workflows and improve data quality in various scientific disciplines.