Abstract

The micropropagation of a number of plant species has been improved through the use of membranes with micropores, resulting in increased acclimatization survival rates. However, there is still a lack of information regarding the application of microporous membranes to the micropropagation of vines. This is due to the low survival of seedlings after acclimatization, which serves as a barrier primarily for breeders who rely on this tool to propagate hybrids and Vitis species spp. In this sense, the purpose of this work was to investigate how the micropropagation of three interspecific hybrids of Vitis spp. was affected by the presence of two in vitro environments and the absence of microporous membranes. through bivariate and morphophysiological analyses. a three-by-two factororial design with a completely random layout two ventilation frameworks with a polypropylene cover with and without the film with two layers of a microporous tape and one of polytetrafluoroethylene) was utilized. It was possible to determine whether the factors (sealings x hybrids) had an effect on the acclimatization phase of the acclimatized vines and interacted with their viability in vitro. Attributing 60% survival to plants grown in lids without membranes, hybrid CH1.2 had no positive interaction with the microenvironment present in membranes. Notwithstanding, the utilization of microporous films decreased CO2 gathering, furnishing an in vitro climate with a lower moistness rate. When acclimatized, the survival of CH5.1 CH1.3 hybrids was 100% due to these favorable environmental conditions. Hence, the utilization of the film benefits micropropagation as well as the endurance of plants when adjusted.