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Hindi Research Article
Microbial Degradation of Reactive Red by Pseudomonas spp. MPS-2
| Maulin P Shah1*, Kavita A Patel1, Sunu S Nair1, Darji AM1 and Shaktisinh Maharaul2 | |
| 1Industrial Waste Water Research Laboratory, Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP), Plot No: 2413/2414, GIDC, Ankleshwar-393 002, Gujarat, India | |
| 2Laboratory of Environmental Bioremediation, Narmada Clean Tech Limited (FETP), Nr. Gujarat Gas, Surti Bhagor, Umarvada Road, Ankleshwar-393001, India | |
| Corresponding Author : | Maulin P Shah Industrial Waste Water Research Laboratory Applied & Environmental Microbiology Lab Enviro Technology Limited (CETP), Plot No: 2413/2414 GIDC, Ankleshwar- 393 002, Gujarat, India Tel: +91-90 999 65504 Fax: +91-2646-250707 E-mail: shahmp@uniphos.com |
| Received June 19, 2013; Accepted August 02, 2013; Published August 04, 2013 | |
| Citation: Shah MP, Patel KA, Nair SS, Darji AM, Maharaul S (2013) Microbial Degradation of Reactive Red by Pseudomonas spp. MPS-2. J Bioremed Biodeg 4:197. doi:10.4172/2155-6199.1000197 | |
| Copyright: © 2013 Shah MP, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Azo dyes are a widespread class of poorly biodegradable industrial pollutants. In anaerobic environments, azo bonds are reductively cleaved yielding carcinogenic aromatic amines, many of which are assumed to resist further metabolism by anaerobes bacteria. The latter compounds generally require aerobic conditions for their degradation. A reactive group of azo dye called C.I: Reactive Red was found to be degraded using pseudomonas spp. MPS- 79 to α-ketoglutaric acid with transient accumulation of 4-aminobenzenesulphonic acid (sulphanilic acid), 4-amino, 3-hydronapthalenesulphonic acid and 4-amino, 5-hydronapthalene 2,7 disulphonic acid as a degradation intermediate in anaerobic facultative batch culture. Colour and Total Organic Carbon (TOC) was successfully removed more than 95% and up to 50% respectively. There is no significant correlation between pH and oxygen depletion since there is slightly change in pH was observed (pH from 7.21 to 7.25) though the anaerobiosis was found developed throughout the experiment (redox potential from 0.7 to 1.6 mV). The anaerobic metabolism of glucose as co-metabolite also shown to provide the electrons required for the initial reductive cleavage of the azo group. This finding suggest that it is possible to mineralize the azo dye in the environment; thereby, avoiding accumulation of toxic intermediates in the water.
