Abstract

Method validation is a critical component in analytical chemistry, ensuring that analytical methods produce reliable and accurate results. This article provides a comprehensive overview of method validation strategies for chemists, detailing the principles, steps, and essential parameters involved in the validation process. Key aspects such as specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness are discussed. The article also highlights the importance of regulatory requirements and guidelines from organizations such as the International Conference on Harmonisation (ICH) and the U.S. Food and Drug Administration (FDA). Finally, practical examples and case studies are presented to illustrate the application of these strategies in various analytical settings, emphasizing the significance of method validation in ensuring the integrity of scientific research and quality control.