Journal of Bioremediation & Biodegradation
Open Access

Research Article

Metabolism and Preferential Utilization of Phenylacetic acid and 4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86

Rahul Shrivastava1, Hemant Purohit2 and Prashant S Phale1*
1Department of Biosciences and Bioengineering, Indian Institute of Technology-Bombay, Powai, Mumbai 400076, India
2Environmental Genomics Unit, National Environmental Engineering Research Institute, Nagpur-440020, India
Corresponding Author :	Dr. Prashant S Phale Department of Biosciences and Bioengineering Indian Institute of Technology-Bombay Powai, Mumbai 400076, India Tel: +91-22-2576 7836 Fax: +91-22-2572 3480 E-mail: pphale@iitb.ac.in
Received March 29, 2011; Accepted June 20, 2011; Published June 21, 2011
Citation: Shrivastava R, Purohit H, Phale PS (2011) Metabolism and Preferential Utilization of Phenylacetic acid and 4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86. J Bioremed Biodegrad 2:120. doi:10.4172/2155-6199.1000120
Copyright: © 2011 Shrivastava R. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at Pubmed Scholar Google

Metabolic pathways for phenylaceticacid (PA) and 4-hydroxyphenylacetic acid (4-HPA) from Pseudomonas putida CSV86 were elucidated. PA grown strain CSV86 cells showed higher oxygen-uptake on PA as compared to hydroxyphenylacetic acids. Detection of phenylacetyl-CoA ligase and absence of 4-hydroxyphenylacetic acid hydroxylase (4HPAH) and 3,4-dihydroxyphenylacetic acid-dioxygenase (3,4-DHPADO) activity supports this observation. 4-HPA grown cells showed oxygen-uptake on 4-HPA and 3,4-dihydroxyphenylacetic acid (3,4-DHPA) but showed significantly lower respiration rates on 2,5-dihydroxyphenylacetic acid and PA. Detection of 4HPAH and 3,4-DHPADO activities support the respiration data. Metabolic studies indicate that strain CSV86 metabolizes PA via phenylacetyl-CoA while 4-HPA via 3,4-DHPA pathway. Glucose grown cells showed lower activity of enzymes and respiration on PA, 4-HPA and 3,4-DHPA, suggesting that pathways are inducible. When grown on double carbon sources such as PA or 4-HPA plus glucose, CSV86 cells showed diauxic growth pattern with oxygen uptake on PA, 4-HPA and 3,4-DHPA in the first log-phase which was reduced during the second log-phase with increase in the respiration rate on glucose. This observation was supported by detection of high 4HPAH and 3,4-DHPADO activity in the first log-phase and glucose-6-phosphate dehydrogenase activity in the second log-phase. These results suggest that strain CSV86 utilizes PA and 4-HPA preferentially over glucose.