Abstract

The cellular composition and barrier integrity of porcine colloid-derived monolayers were investigated to assess the influence of culture media and format. Porcine colloid monolayers were cultured using various media formulations and formats, and their cellular composition and barrier integrity were evaluated using quantitative assays and microscopy techniques. Our results reveal significant alterations in cellular composition and barrier integrity depending on the culture media and format employed. Specifically, certain media formulations were found to promote the growth of specific cell types within the monolayers, while others influenced barrier tightness and integrity. These findings underscore the importance of careful selection of culture media and format in studying and manipulating cellular behavior and barrier properties in porcine colloid-derived monolayers, with potential implications for tissue engineering and disease modeling applications.