Abstract

Ethno pharmacologic connexion: Sijunzi Decoction(SJZD, as a noted classical prescription for the treatment of large intestine cancer(CRC) within the ancient Chinese drugs (TCM), has achieved smart curative effects in clinical follow. However, its specific ingredients and molecular mechanisms continue to be unclear.

Aim of the study: To analyze the effective ingredients and molecular mechanisms of SJZD within the treatment of CRC through network medical specialty technology and experimental validation.

Materials and strategies: First, the TCM Systems medical specialty information and analysis platform information were searched to screen the effective chemical elements of SJZD. Swiss Target Prediction was accustomed predict corresponding potential target genes of compounds. After that, we have a tendency to created a elements and corresponding target network by Cytoscape. at the same time, five sickness databases were accustomed search and filter CRC targets, then we have a tendency to created a drug-disease target proteinprotein interaction (PPI) network. Cytoscape three.7 was used for image and cluster analysis, and Metascape information was used for GO and KEGG enrichment analysis. we have a tendency to histrion the most pathwaytarget network diagram. Autodock vina1.5.6 was applied to molecular arrival for the most compounds and target proteins. after, the potential mechanism of SJZD on carcinoma foretold by network pharmacologic analysis was by experimentation studied and verified in vivo and in vitro.

Results: 144 effective active chemical elements, 897 potential targets, and 2584 CRC target genes were screened out. the quantity of common targets between the SJZD and CRC was 414.3250 GO process things and 186 KEGG signal pathways were obtained when analysis. the most compounds and therefore the target macromolecule had an honest binding ability in molecular arrival. The results of cell and animal experiments showed that SJZD may promote programmed cell death and autophagy of CRC cells through PI3K/Akt/mTOR pathway.

Conclusions: SJZD will treat CRC through multiple elements, multiple targets and multiple pathways. we have a tendency to ab initio unconcealed the effective elements and molecular mechanisms of SJZD within the treatment of CRC, and that we used molecular arrival and experiment for preliminary verification.