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Hindi Research Article
Maximum α-Amylase Production by Molecular and Biochemical Characterized Soil Microorganism
Pushpendra Singh1*, Rajesh Sharma2 and Ravindra Singh11Department of Biological sciences, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidhyalaya, Chitrakoot, Madhya Pradesh, India
2Department of Biotechnology, Veer Bahadur Singh Purvanchal University, Jaunpur, Uttar Pradesh, India
- Corresponding Author:
- Singh P
Department of Biological sciences
Mahatma Gandhi Chitrakoot Gramodaya Vishwavidhyalaya
Chitrakoot, Madhya Pradesh, India
Tel: 07309890484
E-mail: pushpendrasinghbiotech@gmail.com
Received date: June 13, 2017; Accepted date: June 17, 2017; Published date: July 24, 2017
Citation: Singh P, Sharma R, Singh R (2017) Maximum α-Amylase Production by Molecular and Biochemical Characterized Soil Microorganism. J Biotechnol Biomater 7:266. doi:10.4172/2155-952X.1000266
Copyright: © 2017 Singh P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Abstract
The present investigation was undertaken with an aim to produce α-amylase in cost effective way keeping its market potential into consideration. This result obtained from present study prove that Bacillus licheniformis is the maximum α-amylase producing strain isolated from soil under suitable conditions. This study will very helpful for upcoming researchers to uncover the various aspect of enzyme production by microorganism. The present investigation was undertaken with an aim to produce higher amount of amylase with pure compounds in submerged fermentation. To screen the isolates for promising α-amylase producing capability and one of the most promising isolate is further scale up. Amylase constitutes a class of industrial enzymes having approximately 20% of the enzyme market. α-amylase has found its application in a range of industries including food, brewing, distilling industry, textile, paper pharmaceutical and bioconversion of solid waste etc. Microorganism used in present investigation, isolated from rhizospheric regions of giant newar variety of Raphanus sativus grown in district Jaunpur, (Uttar Pradesh) India. A total of 30 natural isolates were scanned for α-amylase activity out of which 20 isolates were biochemically characterized. Based on ribotyping all the isolates were identified and one of the most promising amylase producer, i.e., Bacillus licheniformis was finally selected for further studies. The experiments were carried out in triplicates and mean value was taken. Nutritional source such as carbon and nitrogen sources were optimized for the production of α-amylase in free cell condition. Maximum α-amylase production was observed at 24 h. of incubation. Three different types of media such as Semi-synthetic, complex media I and complex media II tested and various parameters were optimized for enhance the alpha-amylase yield. It was found that identified soil microorganism Bacillus licheniformis is the best strain for α-amylase production and complex media-I gave maximum yield.
