Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Enalapril and Enalaprilat in Human Plasma
*Corresponding Author: Srinivasa Rao, SM Pharmaceuticals SDN BHD, Lot 88, Sungai Petani Industrial Estate, Sungai Petani, Kedah, Malaysia, Tel: +91 8709324537, Email: srinivasaraoj14@gmail.comReceived Date: May 22, 2019 / Accepted Date: Jun 18, 2019 / Published Date: Jun 24, 2019
Citation: Rao S, Chakka G (2019) Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Enalapril and Enalaprilat in Human Plasma. J Anal Bioanal Tech 10: 418.
Copyright: © 2019 Rao S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
A fast, perceptive, and highly discriminating Liquid chromatographic method with Mass spectroscopy was urbanized and validated for simultaneous determination of Enalapril and its major bioactive metabolite Enalaprilat in human plasma. Solid phase extraction process was used for the extraction of analytes from plasma. The chromatographic severance was achieved on Zorbax Eclipse; 150 × 4.6 mm, C18 5 µm column using a solvent system of acetonitrile and 0.1% v/v HCOOH in water with a ratio of 65:35 v/v at a flow rate of 0.8 mL/min. The analytes were detected in a positive ionization by multiple reactions monitoring mode. Mass transitions of m/z 377.10 → 234 for Enalapril, m/z 382.10 → 239.20 for Enalapril D5 and m/z 349 → 206 for Enalaprilat, m/z 354.20 → 211.20 for Enalaprilat D5 were used for quantification in plasma samples. The method exhibited a linear response with a Correlation co-efficient (r2) of >0.99 in the concentration range of 0.502-160.2 ng/mL for Enalapril and 0.506-161.5 ng/mL for Enalaprilat in human plasma. The mean recovery of Enalapril was 91.21% with a precision range of 1.72% to 5.06% and Enalaprilat was 90.85% with a precision range of 1.29% to 3.87%. The proposed method can be utilized for the quantification of Enalapril and Enalaprilat in human plasma in regular bioequivalence studies.
