International Journal of Inflammation, Cancer and Integrative Therapy
Open Access

Research Article

Link between Dimethyl arginine Derivats and Acpa Antibodies in Patients with Rheumatoid Arthritis

¹Department of Rheumatology, University Clinical Centre, Skopje, Republic of Macedonia, Roman, Italy

²Department of Hematology, University Clinical Centre, Skopje, Republic of Macedonia, Roman, Italy

^*Corresponding Author:

Dejan Spasovski
Department of Rheumatology, University Clinical Centre
Skopje, Republic of Macedonia, Rome, Italy
Tel: +389023147147
E-mail:drspasovski@yahoo.co.uk

Received date: November 04, 2014; Accepted date: December 06, 2014; Published date: December 10, 2014

Citation: Spasovski D and Sotirova T (2014) Link between Dimethyl arginine Derivats and Acpa Antibodies in Patients with Rheumatoid Arthritis.Interdiscip J Microinflammation 1:122. doi: 10.4172/2381-8727.1000122

Copyright: © 2014 Spasovski D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Introduction: When establishing new diagnostic method for detection of the endothelial dysfunction in Rheumathoid arthritis (RA), it is necessary to compare the diagnostic values with other laboratory variables.

Aim: The aim of this study is to determine the association between the asymmetric dimethylarginine (ADMA) and anti-cyclic citrullinated peptide antibodies (ACPA, Anti-CCP2) of the second generation in RA and to explain their connection in the process of citrulination of autoantigens in RA. Also, to determine the association between ADMA and the acute phase reactants: Rheumatoid factor (RF), C-reactive protein (CRP), as well as the index of disease intensity (DAS28) in early diagnosis in non-treated RA patients and to determine whether the amount of ADMA changes in the course of the evolution of disease.

Methods: Using the ELISA method (DLD-Diagnostika-GMBH for ADMA and Bio Systems S.A. Reagens and Instruments Costa Brava 30, Barselona, Spain), the sera of 70 subjects have been examined (35 RA not-treated, 35 healthy control group).

Results: Of 35 patients with RA, presence of ADMA was detected in 13 patients (37.14%); RF was present in 17 patients (48.57%), while anti-CCP2 antibodies were present in 23 patients (65.71%). Of 18 patients who were RF negative, ADMA was detected in 9 patients (50%), while 11 patients (31.43%) were anti-CCP2 positive. In 17 RF positive patients with RA, ADMA was detected in 4 patients, while 12 patients (34.28%) were anti-CCP2 and RF positive. In the healthy control group 8 patients (22.85%) showed ADMA positivity. There was moderate correlation between ADMA and anti-CCP2 antibodies in the group of patients with RA (r=0.34).

Conclusion: There was an association between ADMA and anti-CCP2 antibodies of the second generation in patients with RA.

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