Abstract

CD34, a cell surface marker expressed on hematopoietic stem and progenitor cells, is crucial for various research and clinical applications in the field of hematology. Several methods have been developed for the isolation of CD34+ cells from peripheral blood mononuclear cells (PBMCs), each with its advantages and limitations. In this study, we compared three commonly used methods for CD34 isolation: magnetic-activated cell sorting (MACS), anti-CD34 antibody-based selection, and manual isolation by gradient centrifugation. PBMCs were obtained from healthy donors, and CD34+ cells were isolated using each method. The efficiency, purity, and viability of isolated CD34+ cells were evaluated, along with their ability to form hematopoietic colonies in culture. Our results provide insights into the strengths and weaknesses of each isolation method, aiding researchers and clinicians in selecting the most appropriate technique for their specific needs.