Abstract

Oil pollution is currently a global problem. However, microorganisms that can consume petroleum oil and utilise hydrocarbon for their development, nourishment, and metabolic activities are enriched in oil-contaminated environments. In the current work, bacteria from oil-contaminated soil were isolated using the enrichment culture method using Minimal Salt media supplemented with diesel oil and burnt engine oil as the only carbon source. The isolated bacteria were examined for morphology and biochemistry, and they were then identified using a molecular tool and the cycle sequencing approach. Three isolates were identified as being gram-negative, cocci-shaped, and closely related to Pseudomonas species, Acinetobacter species, and Enterobacter species, respectively, according to 16S rRNA sequence analysis. The ideal growth environment was at and temperature. Every isolate was vulnerable.