Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

Isolation and Quantitative Determination of New Tuberculostatic 1,2,4-Triazole Derivative in Urine and Plasma Samples

¹Department of Analytical Chemistry, Medical University, Chodzki 4a, 20-093 Lublin, Poland

²Department of Organic Chemistry, Medical University, Chodzki 4a, 20-093 Lublin, Poland

³Department of Biochemistry, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland

*Corresponding Author:

Jolanta Flieger
Department of Analytical Chemistry, Medical University
Chodzki 4a, 20-093 Lublin, Poland
Tel: +48 81535-73-50
E-mail: j.flieger@umlub.pl

Received date: September 09, 2014; Accepted date: September 25, 2014; Published date: September 29, 2014

Citation: Tatarczak-Michalewska M, Flieger J, Wujec M, Swatko-Ossor M (2014) Isolation and Quantitative Determination of New Tuberculostatic 1,2,4-Triazole Derivative in Urine and Plasma Samples. J Anal Bioanal Tech 5:206 doi: 10.4172/2155-9872.1000206

Copyright: © 2014 Tatarczak-Michalewska M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

A novel series of 1,2,4-triazole derivatives, have been synthesised. All synthesised compounds were screened for their antimycobacterial activity against Mycobacterium tuberculosis and antimicrobial activities against various bacteria and fungi. Among studied derivatives, 4-methyl-1-morpholinmethyl-2,4-dihydro-3H-1,2,4-triazol-3-thione was found as the most active, demonstrating inhibition of mycobacterial growth of Mycobacterium H37Ra at the same concentration level as Streptomycin. The quantitative analysis of this compound in the urine and serum samples was elaborated on. Quantification was performed by reversed-phase high-performance liquid chromatography (RP–HPLC) using C18 column with a mobile phase consisting of methanol and water (5:95) at flow rate of 1 ml/min. The chromatograms were recorded at 245 nm at 21°C. The chromatographic peak identification was based on the comparison the retention times and UV spectra of isolated analyte and synthetized standard. Moreover, different sample pre-treatment methods such as deproteinization and solid-phase extraction (SPE) were compared. The highest analyte recoveries ranging from 78,7% to 90,7% were obtained for sample deproteinized by 6% HClO₄.

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