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Isolation and Characterization of Gasoline-Degrading Yeasts from Refined Oil-Contaminated Residues
| Goulart GG1, Coutinho JOPA1, Monteiro AS2, Siqueira EP3 and Santos VL1* | ||
| 1 Department of Microbiology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte – MG, C.P. 486, 31270-901, Brazil | ||
| 2 Laboratório de Pesquisa em Microbiologia – Faculdade de Ciências da Saúde, Universidade Vale do Rio Doce, Governador Valadares, MG, Brazil | ||
| 3 Laboratório de Química de Produtos Naturais, Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, C.P. 30190- 002, Belo Horizonte, MG, Brazil | ||
| Corresponding Author : | Vera Lúcia dos Santos Department of Microbiology ICB-UFMG, C.P. 486, 31270-901 Belo Horizonte, Brazil Tel: 5534092501 Fax: 55(31)34092730 E-mail: vlsantos@icb.ufmg.br |
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| Received November 04, 2013; Accepted February 03, 2014; Published February 08, 2014 | ||
| Citation: Goulart GG, Coutinho JOPA, Monteiro AS, Siqueira EP, Santos VL (2014) Isolation and Characterization of Gasoline-Degrading Yeasts from Refined Oil-Contaminated Residues. J Bioremed Biodeg 5:214. doi:10.4172/2155-6199.1000214 | ||
| Copyright: © 2014 Goulart GG, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
Hydrocarbon-degrading yeasts were isolated from three sites contaminated with products of refined petroleum originating from an oily residue treatment Brazilian company. A selective enrichment technique was used by supplementing medium with gasoline, which resulted in eleven isolates; two identified as Rhodotorula mucilaginosa, one as Rhodosporidium diobovatum, four as Meyerozyma (Pichia) guilliermondii, two as Wickerhamia sp., and two as Meyerozyma sp. The strains were evaluated for their growth capacity in medium containing 1% (v/v) gasoline, kerosene or lubricating oil as the only carbon source; the largest values for cellular biomass and growth rates (μ) were observed with gasoline supplementation. The strains were tolerant to aromatic (toluene and xylene) and aliphatic (hexene and n-heptane) compounds, which are part of the composition of gasoline, at concentrations up to 30 mM toluene (0.3% v/v), 20 mM xylene (0.25% v/v), 80 mM n-heptane (1.17% v/v) and 100 mM hexane (1.33% v/v). The R. mucilaginosa S47 and Meyerozyma sp. SP1 strains showed the greatest degradation percentages of gasoline, and have the potential to be used in the bioremediation of gasoline-contaminated environments.
